The possible effect of human follicular fluid (hFF) on the growth ands development of fertilized oocytes and embryos is important because the fallopian tubes are exposed to FF after follicular rupture and the processes of fertilization and embryo
cleavage occur inside the fallopian tubes. Previously, it was suggested that human FF might adversely affect on the development of early mouse embryos.
In order to investigate the effect of hFF on the development of embryos, early mouse embryos were cultured in media containing various protein sources as bovine serum albumin (BSA), fetal cord serum (FCS) and FF. And we evaluated the development
of
early mouse embryos in terms of the morphology, cleavage rate, and cell count of blastcysts.
There were no significant difference in the morula and blstocyst formation rates of 2-cell mouse embryos cultured in the media containg three different protein sources and three different concentrations of FF.
The blastocyst formation rate of 1-cell mouse embryo cultured in FF group was significantly higher than that cultured in BSA group (P<0.05).
The morula and blastocyst formation rates of 2-cell mouse embryos of the group cultured in the media containing FF were comparable with those of other two groups, in addition, the cell count of blastocyst of FF group in the 2-cell embryo culture
was
higher than those of BSA group and HCS group (P<0.01), and this finding was also noted in 1-cell embryo culture.
There was no difference in the morula and blastocyst formation rates of the 2-cell mouse embryos cultured in the media containing different concentrations of FF.
These results suggest that mature human follicular fluid has no inhibitory on the development of early mouse embryos even in high concentration and may be a good protein source which is positively associated with the development of mouse embryos
in
vitro especially in 1 cell embryo culture.
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